Extended Data Fig. 8: Secondary structure prediction of preE-miR-21 and G38U mutant by Mfold and MC-Fold.
From: Visualizing a protonated RNA state that modulates microRNA-21 maturation
a, M-fold predicted secondary structure of preE-miR-21, which contains G-U base pairs as observed in NMR data of the ground state. b, MC-Fold predicted secondary structures of preE-miR-21. Shown are the top 5 lowest-energy structures, ranked with ∆G relative to the lowest predicted structure. The A22-G38 base pair is predicted in all MC-Fold structures. c, M-fold predicted secondary structure of preE-miR-21 G38U mutant. d, MC-Fold predicted secondary structures of preE-miR-21 G38U mutant. Shown are the top 5 lowest-energy structures, ranked with ∆G relative to the lowest predicted structure. Both M-fold and MC-Fold predict the same lower stem structure of preE-miR-21 G38U mutant, which is also consistent with NMR data.
