Extended Data Fig. 10: Single-turnover pre-miR-21 processing with 10-fold, 25-fold, and 250-fold excess Dicer enzyme.
From: Visualizing a protonated RNA state that modulates microRNA-21 maturation
a, Single-turnover processing assays of recombinant human Dicer (500 nM) with pre-miR-21 at 50, 20, and 2 nM concentrations at pH 7.77 and 35 °C. b, Quantification of time-dependent Dicer processing of wild-type pre-miR-21 using ImageQuant. Solid lines represent best fits to a single exponential equation as described in Methods. Shown are mean values and standard deviations (s.d.) from n = 3 independent assays.
