Extended Data Fig. 3: Comparison of GS, ES, mutant carbon chemical shifts.
From: Visualizing a protonated RNA state that modulates microRNA-21 maturation
Shown are differences between the observed chemical shifts (black) and the chemical shifts of GS (blue) and ES (red) extracted from 13 C R1ρ RD profiles at pH 6.45, G38U mutant at pH 6.45, and preE-miR-21 at pH 4.81. The apparent discrepancies between ES base carbon chemical shifts of A-C2/C8s and C-C5/C6s and their corresponding chemical shifts at pH 4.81 are likely due to intrinsic protonation at A-N1s and C-N3s at pH 4.81.
