Fig. 1: Design and validation of the VH-phage library.
From: Bi-paratopic and multivalent VH domains block ACE2 binding and neutralize SARS-CoV-2
a, Three-dimensional (3D) surface representation (left) of the VH-4D5 parental scaffold (PDB 1FVC) and a cartoon diagram (right), where individual CDRs are annotated in color with the designed loop length variations according to the Kabat nomenclature31. b, NGS analysis of the longest H3 loop (X = 16), showing that the expected global amino-acid frequencies are comparable to the designed frequencies. The gray shaded region denotes the 95% confidence interval. c, Representative NGS analysis of the longest H3 loop (X = 16), showing that the positional frequency distribution matches the designed frequencies. Position 1 refers to residue 95 (Kabat definition). Data for the other CDR H3 lengths are reported in Supplementary Fig. 2. d, NGS analysis of unique clones, showing that all H3 lengths are represented in the pooled VH-phage library.
