Extended Data Fig. 6: BRD4 occupancy in response to treatment with NVS-BET-1 is highly dynamic.
From: BET bromodomain inhibitors regulate keratinocyte plasticity
(a) Spearman correlation of BRD4 peaks genome-wide in human keratinocytes after treatments as indicated, averaged across replicates per condition. (b) Normalized BRD4 ChIP-seq signal centered on peak summit (± 1.5 kb) at genomic loci that displayed significant change in occupancy signal in response to 125 nM NVS-BET-1 compared to DMSO control at 6 h (Differential analysis was performed using DiffBind with DESeq2 (abs(log2FC) > 1 & p-value < 0.01). Peaks are clustered by direction of fold-change (occupancy ‘Down’ versus ‘Up’, as labeled in side heat map on right) and ranked by signal strength with functional annotation based on NHEK ChromHMM signal.
