Extended Data Fig. 6: BC1618 in vivo toxicity studies.
From: A Fbxo48 inhibitor prevents pAMPKα degradation and ameliorates insulin resistance
(a-d) C57BL/6 mice were given BC1618 in drinking water at 15 mg/kg/d (low) and 30 mg/kg/d (high) doses for 3 months. Mice were then euthanized, and plasma samples were collected and assayed for markers of cytotoxicity. Alanine aminotransferase (ALT) activity, creatine kinase activity, lactate dehydrogenase (LDH) content, and creatinine content were measured following the manufacturer’s protocol. e, Major organs were collected and processed for H&E staining; scale bar: 100 μm. Data represented mean +/- SEM. Data points represent n=7–8 independent mice per group, and p-values are indicated, as calculated by one-way ANOVA with Dunnett’s test of multiple comparisons (A-D).
