Extended Data Fig. 4: BC1618 off-targeting kinase screen.
From: A Fbxo48 inhibitor prevents pAMPKα degradation and ameliorates insulin resistance
The inhibitory activities for BC1618 (10 µM) against 51 kinases (ABL, CSK, EGFR, EPHA2, EPHB4, FGFR1, FLT3, IGF1R, ITK, JAK3, KDR, LCK, MET, PDGFRα, PYK2, SRC, SYK, TIE2, TRKA, TYRO3, AKT1, AMPKα1/β1/γ1, AMPKα2/β1/γ1, AurA, CaMK4, CDK2/CycA2, CHK1, CK1ε, DAPK1, DYRK1B, Erk2, GSK3β, HGK, IKKβ, IRAK4, JNK2, MAPKAPK2, MST1, NEK2, p38α, p70S6K, PAK2, PBK, PDK1, PIM1, PKACα, PKCα, PKD2, ROCK1, SGK, TSSK1) were measured and percentage inhibition of kinase activity was plotted. 50% change of kinase activities is indicated by a red line cut off line (CarnaBio) (Data are the mean of two biological replicates).
