Extended Data Fig. 3: Derivatized thermal melt curves for compound-treated mLYPLAL1.
From: Discovery of small-molecule enzyme activators by activity-based protein profiling
Temperature-dependent fluorescence shifts in purified mLYPLAL1 incubated with increasing concentrations of 4 and 12. Increased protein flexibility is noted upon compound interaction. In contrast, an inactive compound, 80, does not shift the derivatized thermal melt curve of mLYPLAL1. Representative results from three independent experiments; similar results were obtained in all experiments.
