Extended Data Fig. 2: The synthetic condensates are not membrane-bound.
From: Designer protein assemblies with tunable phase diagrams in living cells
a, Transmission electron microscopy (TEM) micrograph of fixed and sectioned yeast shows a condensate formed by our minimal system, in the cytoplasm. b, The yellow arrow points to one of several 10 nm gold-labeled anti-GFP antibodies, confirming the identity of the designed compartments. White arrows highlight the lack of membrane surrounding the compartment. c, Scanning electron microscopy micrograph of cells frozen at high-pressure and cryo-fractured reveals the mosaic of amorphic cytoplasm. The region outlined by white carets exhibits a distinct ultrastructure d, Increased magnification of a suspected condensate within the cytoplasm, outlined with white carets. This ultrastructure has no visible membrane. Scale bar 1 µm. We did not carry independent biological replicates of these electron microscopy experiments.
