Extended Data Fig. 4: Conservation of the TcmC binding site.
From: Tetracenomycin X inhibits translation by binding within the ribosomal exit tunnel
a, In wild-type U2609 basepairs with A752; (b-d) in silico mutations of TcmX resistant mutations (U2609G, U2609C and U2609A) which can no longer basepair with A752 and due to possible clashing have to adopt a different conformation. e, In wild-type U1752 base-pairs with U2586, while in the resistance mutations (f-i) the previously base-paired nucleotides will have to adapt a different conformation to avoid clashing, possibly obstructing the TcmX binding site. j, In E. coli (grey) TcmX stacks upon a U–U basepair U1782-U2586; in T. thermophilus (light blue) (PDB 4V9A)21 it is a C–C base-pair C 1814:C2599. k, Bacterial 23 S (above) and eukaryotic 28 S (below) rRNA alignments of select organisms within the vicinity of the U1782 and U2586 (E. coli numbering) in eubacteria and U3644 and U4532 (H. sapiens numbering).
