Extended Data Fig. 4: Turnover of genomic mdC and its derivatives under priming conditions (related to Fig. 2).
From: Active turnover of genomic methylcytosine in pluripotent cells
a, b, Independent biological replicate of the experiment reported in Fig. 2c,e. a, Global profiles of the indicated labelled cytosine derivatives and dT+12 in the genome of mPSCs under priming conditions (serum/CR) upon chasing of m+4Met. Each panel shows fold change values relative to the highest level for each modification from an individual experiment. Dashes represent single technical replicate measurements (n = 3) from single samples. Full lines lines represent first order decay curves fitted to mean values (in the case of f+2dC t = 0 h was excluded from curve fitting). b, Absolute levels of genomic m+4Met from the same samples as in a. Black squares represent single technical replicate measurements (n = 3) from single samples and bars show their mean values.
