Extended Data Fig. 9: Durability of LYTAC-mediated degradation in HEP3B cells.
From: LYTACs that engage the asialoglycoprotein receptor for targeted protein degradation
a, HEP3B cells were treated with 10 nM Ctx conjugates, then washed with PBS 3 times, and were incubated in fresh media for 6, 24, 48 h. EGFR levels were measured by western blot. 100 ng/ml of EGF was included as a control. b, Quantification of EGFR levels with and without wash-off following treatment with Ctx conjugates. Values are the average of three independent experiments ± SEM. P values were determined by unpaired two-tailed t-tests.
