Extended Data Fig. 1: Cloning of silent double barcode (SDB) regions.
From: Genetically encoded multivalent liquid glycan array displayed on M13 bacteriophage
a, Location of SB1 and SVEK Peptide region (SB2) on initial template. b, Primer 1 and 4 were used to convert M13KE QFT*LHQ to M13KE SDBlib QFT*LHQ. c, Frequency plot analysis of the top leader region of 100 unique sequences observed in deep sequencing of M13KE SDBlib vector; notably nucleotide C was completely suppressed in position 8 even though it was possible by design (CTN codon in Primer 1); d, Distribution and cumulative distribution of the unique sequences in deep sequencing of M13KE SDBlib vector. From 6144 possible sequences the most abundant ~200 unique sequences dominated 90% of the available diversity. e, Primer 5 and 6 were used to convert M13KE SDB QFT*LHQ to M13KE SDB SVEK library. f, Summary of degenerate sites in M13KE SB1 and SVEK Peptide (SB2) regions. g, Location of Illumina Sequencing Primers. h, Sequences of the Illumina sequencing primers.
