Extended Data Fig. 8: Cell states from CD8+ T cells residing in intermingled tumor areas.
From: Single-cell analysis of regions of interest (SCARI) using a photosensitive tag
(a) Confocal image of coculture of Ag-specific CD8+ T cells with tumor cell islands in which Ag+ tumor cell areas (green) and Ag− tumor cell areas (magenta) are intermingled. Scale bar represents 2 mm. Representative of 2 independent experiments. (b) Quantification of T cell states in uncaged αCD8D−1-PsT labeled CD8+ T cells from Ag+ and Ag− areas in a mixed tumor culture as shown in Extended data Fig. 8a. Bar plots show the quantification of T cell states as defined in Fig. 4a of uncaged CD8+ T cell populations from samples in which either Ag+ or Ag− areas were uncaged (252 and 236 cells, respectively). Tumor cells are excluded from the analysis. Two-tailed Chi-square test was performed (**** indicates p < 1*10−15). Data shown were obtained in a single experiment.
