Extended Data Fig. 6: Full time course data of LbuCas13a and LbuCas13a-TtCsm6 detection experiments in Fig. 3b,c.
From: Accelerated RNA detection using tandem CRISPR nucleases
a) Raw fluorescence signal from LbuCas13a-mediated cleavage of a fluorescent reporter in the presence of 0-125 copies/µl (cp/µl) of B.E.I. SARS-CoV-2 RNA. LbuCas13a is complexed with eight crRNAs targeting the SARS-CoV-2 genome (604, 612, 542, 546, 564, 569, 588, 596). The mean raw fluorescence values (arbitrary units, AU) ± s.e.m. are plotted as lines with error bars (n = 3). These data are used for detection analysis in Fig. 3b at 20 min and 118 min. b) Raw fluorescence signal from an LbuCas13a-TtCsm6 detection assay containing 0-125 cp/µl B.E.I. SARS-CoV-2 genomic RNA. The same crRNAs were used as in a. The mean raw fluorescence values (arbitrary units, AU) ± s.e.m. are plotted as lines with error bars (n = 3). c) Graph of the data in a, but with measurements normalized to the fluorescence at t = 6 min. The mean normalized fluorescence values (F/Ft=6) ± S.E.M. are shown as lines with error bars (n = 3). d) Graph of the data in b, but normalized to the fluorescence at t = 6 min. The mean normalized fluorescence values (F/Ft=6) ± s.e.m. are shown as lines with error bars (n = 3). These data were used in Fig. 3c for detection analysis at 20 min and 118 min. e) Same dataset as in c, but zoomed to the first 30 min of the LbuCas13a assay. f) Same dataset as in d, but zoomed to the first 30 min of the LbuCas13a-TtCsm6 assay.
