Extended Data Fig. 4: Binding and modification of ring-containing precursors.
From: Molecular mechanism underlying substrate recognition of the peptide macrocyclase PsnB
a,b, Affinity of ring-containing MPs (MP-1H2O, the single-ring intermediate; MP-2H2O, the double-ring product) to PsnB was determined by fluorescence anisotropy without nucleotide (a) or with 1 mM ADP (b). c, ATPase activity of PsnB was measured with different concentrations of MP or the single-ring intermediate (MP-1H2O). Data are presented as dot plots with mean ± 1 SD (n = 3 independent experiments; a-c) and fitted to a hyperbolic equation (a-c).
