Extended Data Fig. 8: CryoEM data processing for SP6-11-NK1R-miniGs/q70 complex.
From: Selective G protein signaling driven by substance P–neurokinin receptor dynamics
(a) Representative micrograph of 3878 collected micrographs. Scale bar, 50 nm. (b) 2D-class averages for SP6-11-NK1R-miniGs/q70 complex. (c) A flowchart representation of the processing pipeline used for structural determination of the SP6-11-NK1R-miniGs/q70 complex. CTF Estimation, 2D classification and all 3D classification jobs with alignment were performed with cryoSPARC. 3D classification without alignment was performed with RELION using a mask encompassing only the receptor transmembrane and final focused refinements were performed with cisTEM. Focused refinement masks are shown as red mesh. GS-FSC was calculated from a cryoSPARC Local Resolution job using the focused refinement mask encompassing the entire SP6-11-NK1R-miniGs/q70 complex. A viewing distribution plot was generated using scripts from the pyEM software suite and visualized in ChimeraX. Directional FSC curves (dFSC) are shown in purple lines and were determined as previously described in Dang, S. et al. Nature 552, 426-429 (2017).
