Extended Data Fig. 1: Biochemistry of active-state NK1R–miniG protein complexes. | Nature Chemical Biology

Extended Data Fig. 1: Biochemistry of active-state NK1R–miniG protein complexes.

From: Selective G protein signaling driven by substance P–neurokinin receptor dynamics

Extended Data Fig. 1

(a) Size-exclusion chromatography of SP-bound NK1R, NK1R-miniGs/q70 and NK1R-miniGs399 shows an increase in the fraction of monomeric receptor species for NK1R-miniG fusion proteins. Size-exclusion chromatography traces and Coomassie stained SDS-PAGE gels of purified (b) SP-bound NK1R-miniGs/q70 complex, (c) SP-bound NK1R-miniGs399 complex, and (d) SP6-11-bound NK1R-miniGs/q70 complex. Uncropped versions of Coomassie stained SDS-PAGE gels are provided in Supplementary Fig. 1.

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