Extended Data Fig. 4: Workflow for cryo-EM image analysis and validation for ammocidin-bound and apoptolidin-bound yeast ATP synthase.
From: Apoptolidin family glycomacrolides target leukemia through inhibition of ATP synthase
Example micrographs (a, c), 2D class average images (b, d), and workflow for obtaining maps of the F1 regions (e, f) for ammocidin-bound, and apoptolidin-bound ATP synthases, respectively. Corrected Fourier shell correlation curves after gold-standard refinement g, local resolution maps h, and orientation distribution plots i, are shown for the maps of the F1 regions of the ammocidin-bound, apoptolidin-bound, and inhibitor-free data sets. Alternative conformations of ammocidin (j) and apoptolidin (k) atomic models.
