Fig. 1: Mandi, a new CIP.
From: Mandipropamid as a chemical inducer of proximity for in vivo applications
a, Chemically induced protein proximity to control interactions between proteins of interest A and B. b, Chemical structure of Mandi. c, Live-cell confocal microscopy of COS-7 cells cotransfected with pLYN-mCherry-PYRMandi and enhanced green fluorescent protein peGFP-ABI before and 2 min after Mandi addition (100 nM); data are representative of seven cells. d, Live-cell confocal microscopy of COS-7 cells transfected with pvimentin-mNeonGreen-PYRMandi-IRES-Halo-ABI and labeled with HaloTag ligand-SiR (HTL-SiR). Images were acquired before and 5 min after Mandi addition (50 nM); data are representative of 20 cells. The scale bars in c and d represent 10 µm. See Extended Data Fig. 2 for single-channel images.
