Extended Data Fig. 6: Nanobody assisted targeting of chemically induced protein proximity.
From: Mandipropamid as a chemical inducer of proximity for in vivo applications
a, Schematic illustration (a) of nanobody assisted targeting of chemically induced protein proximity. b, HeLa cells stably expressing LifeAct-GFP were transfected with antiGFP-nanobody-PYRMandi and mCherry-ABI fusions. Confocal images acquired before and 5 min after addition of Mandi. Pearson’s correlation coefficients indicated in images. Representative data for 30 cells in 2 independent experiments. Scale bar: 20 µm. c, Line profiles of GFP and mCherry signal intensity in ROI (yellow box) before and after addition of Mandi.
