Extended Data Fig. 8: Reversible and dynamic protein shuttling between mitochondria and keratin in living cells.
From: Mandipropamid as a chemical inducer of proximity for in vivo applications
COS-7 cells were co-transfected with Keratin-mNeonGreen-PYRMandi-IRES-Halo-ABI and TOM20-SNAPf-PYL. Halo- and SNAP-tag domains were stained with HTL-SiR and TMR-Star 2 h prior to imaging. a, Representative confocal fluorescence microscopy images of four-step shuttling between cytosol, mitochondria and keratin over time. Upper row shows dynamic receiver localization, middle row receptor localizations as references, lower row respective merges. Images acquired at t0, 10 min after addition of ABA-AM (200 nM), 25 min after addition of revABA (20 μM), 10 min after addition of Mandi (200 nM). Scale bar 20 µm. b, Pearson correlation coefficients (PCC) between images receiver and respective receptor channel images at indicated time points for four-step shuttling between cytosol, mitochondria and keratin as shown in (a). 4 cells from 1 experiment. Mean±SD across all cells indicated by dark circles and error bars.
