Extended Data Fig. 2: Mandi-induced protein translocation to different subcellular targets.
From: Mandipropamid as a chemical inducer of proximity for in vivo applications
Live-cell fluorescence microscopy of COS-7 cells transiently transfected with receiver domains targeted to subcellular targets and cytosolic receiver domain. Images were acquired by epi- (a) or confocal (b-d) fluorescence microscopy before and 2 min (c) or 5 min (a,b,d) after addition of Mandi at indicated final concentrations. Transfection with pTOM20-mCherry-PYRMandi-IRES-EGFP-ABI (a), pKeratin-mNeonGreen-PYRMandi-IRES-Halo-ABI (b), pLYN-mCherry-PYRMandi and peGFP-ABI (c), Vimentin-mNeonGreen-PYRMandi-IRES-Halo-ABI (d). Halo-ABI was labeled with HTL-SiR. Scale bars 20 µm. Representative data for 6–20 cells in 1–2 independent experiments.
