Extended Data Fig. 3: Quantitative characterization of Mandi via control of transcriptional activation and RICS.
From: Mandipropamid as a chemical inducer of proximity for in vivo applications
a, Schematic illustration of CIP-induced luciferase expression related to Fig. 2e. b, Representative cross-correlation functions of YFP and mCherry signal in COS-7 cells transiently transfected with indicated constructs. Left: negative (YFP + mCherry) and positive (YFP-mCherry fusion) controls. Right: mCherry-ABI vs. YFP-PYRMandi before (top) and after (bottom) addition of 500 nM Mandi. Binding efficiency for shown mCherry-ABI vs. YFP-PYRMandi after Mandi addition cross-correlation: 73%.
