Extended Data Fig. 1: RT-DNA sequencing prep.
From: Precise genome editing across kingdoms of life using retron-derived DNA
a. Schematic of the sequencing prep pipeline for RT-DNA. b. Representative image of a PAGE analysis showing the addition of nucleotides to the 3’ end of a single-stranded DNA, controlled by reaction time. The experiment was repeated twice with similar results. c. Alternate analysis of the RT-DNA for the a1/a2 length library, using a TdT-based sequencing preparation. Related to Fig. 2.
