Extended Data Fig. 2: RT-DNA production in eukaryotic cells.
From: Precise genome editing across kingdoms of life using retron-derived DNA
a. Representative image of a PAGE analysis of Eco1 and Eco2 RT-DNA isolated from yeast. The ladder is shown at a different exposure to the left of the gel image. The experiment was repeated twice with similar results. b. Enrichment of the Eco1 RT-DNA/plasmid template when uninduced compared to a dead RT construct. Closed circles show each of three biological replicates, with red for the dead RT version and black for the live RT. c. Identical analysis as in b, but for Eco1 in HEK293T cells. Related to Fig. 3.
