Extended Data Fig. 4: Imprecise editing profile of the yeast ADE2 locus.
From: Precise genome editing across kingdoms of life using retron-derived DNA
a. Percent of ADE2 loci with imprecise edits or sequencing errors at 24 and 48 hours. Closed circles show each of three biological replicates, with black for the wt a1/a2 length and green for the extended a1/a2 (two extended versions, v1 and v2). Induction conditions are shown below the graph for the RT and Cas9. b. Breakdown of the data in a. by type of edit/error. c. Imprecise edits and sequencing errors found in all data sets, ranked by frequency. Above the graph are the wt ADE2 locus and intended precise edit. On the Y axis are the imprecise edits and sequencing errors found. X axis represents count of each sequence in all data sets. Related to Fig. 4h.
