Extended Data Fig. 10: Comparison of Squash and iSpinach recognition of DFHBI-1T.
From: The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold
a, Cartoon representation of the fluorophore binding site of iSpinach (PDB:7L0Z; ref. 5). Orange dashed lines denote hydrogen bonds. Residue numbers correspond to those of Spinach (ref. 6). b, Fluorescence lifetimes (Methods) of DFHBI-1T bound to Squash and iSpinach (green squares and cyan triangles, respectively; means ± s.d., n = 3). Black circles, instrument response function (IRF).
