Extended Data Fig. 5: GCN2 is sufficient for neratinib induced eIF2α phosphorylation.
From: GCN2 kinase activation by ATP-competitive kinase inhibitors
a, Schematic of recombinant GCN2 electroporation rescue. b, immunoblot of eIF2α phosphorylation for SKMG3 cells electroporated with recombinant GCN2 then treated with 675 nM neratinib for 4 hours. Western blots were performed with a minimum of n = 2 biological replicates with similar results obtained each time. c, image quantification of eIF2α phosphorylation, normalized to vinculin.
