Extended Data Fig. 3: Constitutive activation of eIF2α induces cell death.
From: GCN2 kinase activation by ATP-competitive kinase inhibitors
a, Immunoblot of SF268 cells were transduced with doxycycline (dox) inducible wild-type (WT) or constitutively active (S51D) eIF2α and treated with 5 µg/mL dox for indicated times (upper band, tagged recombinant eIF2α). Western blots were performed with a minimum of n = 2 biological replicates with similar results obtained each time. b, effect of dox induced WT or S51D eIF2α on cell growth. Data are presented as mean values ± SD of n = 3 biologically independent samples.
