Extended Data Fig. 2: Activity analysis of BpLGDH. | Nature Chemical Biology

Extended Data Fig. 2: Activity analysis of BpLGDH.

From: Metabolism of a hybrid algal galactan by members of the human gut microbiome

Extended Data Fig. 2: Activity analysis of BpLGDH.

a, screen of BpLGDH activity against various monosaccharides using NAD+ as a co-factor. n = 3 independent reactions were performed with each replicate shown along with the mean ± standard deviation. b and c show the kinetics using NAD+ and NADP+, respectively, as a co-factor when excess L-galactose is used as a substrate. d, the kinetics of L-galactose oxidation using excess NADP+ as a co-factor. The data is shown as the mean ± standard deviation of n = 3 independent reactions (see Supplementary Fig. 1 for independent data points). The solid line shows the best fit line to the Michaelis-Menton equation.

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