Extended Data Fig. 1: Metrics of the modified organoid HTS system.
From: Organoid screening reveals epigenetic vulnerabilities in human colorectal cancer
a, The growth of organoids in Matrigel-embedded culture, static floating culture and floating culture in a rotating vessel. The number of cells in each culture condition was counted on day 0, 2, 5, 7 and 9. n = 3 technical replicates for each condition. b, Doubling time of organoids in the indicated culture conditions. The doubling time was calculated by linear regression of the day of harvesting and logarithmic average cell numbers from day 0 to day 7. c, Comparison of organoid growth metrics measured using confocal fluorescence imaging, ATP abundance and bright-field imaging. Each dot indicates one well. Organoid viability is shown as the relative to the mean of vehicle-treated control. Note that the viability of organoids treated with mTOR inhibitors appear lower in the fluorescence measurement than in the ATP-based assay. d, Comparison of the Z’-factors in conventional and refined culture conditions.
