Extended Data Fig. 6: Supplementary data for Fig. 2h.
From: An infection-induced oxidation site regulates legumain processing and tumor growth
Western blot analysis of legumain, HA-tagged ubiquitin, and Myc-tagged TRAF6 before (left, input) and after (right, IP) anti-FLAG IP of lysates from H. pylori-infected and uninfected HEK293T cells transiently transfected with FLAG-tagged LGMN, HA-tagged UBB, and/or Myc-tagged TRAF6. Cells were infected with H. pylori G27MA at MOI 25 for 15 h followed by a 3-h incubation in serum-deficient medium. Western blot analyses were performed three times with consistent results.
