Extended Data Fig. 3: SalC activity assay with diffusible substrates.
From: Enzymatic assembly of the salinosporamide γ-lactam-β-lactone anticancer warhead
a, Reaction scheme depicting chemoenzymatic synthesis and subsequent SalB-PCP acylation assay to generate (15). b, LCMS chromatograms (BPCs and EICs m/z 266.18) of SalC activity assay with diffusible substrates shown in part (a) including pantetheine-activated (13), CoaA product-activated (19), CoaD product-activated (20), CoA activated (14), and carrier protein-activated substrate (15). Substrates were activated via in vitro CoA enzyme biosynthesis. All SalC activity assays contained apo-SalB-PCP as well.
