Extended Data Fig. 6: NorA-Fab binding interfaces.
From: Structural basis for inhibition of the drug efflux pump NorA from Staphylococcus aureus
a, b. Detailed views of CDRH3 interactions in the substrate-binding pocket of NorA (rainbow) for Fab25 (panel (a); pink) and Fab36 (panel (b); blue). The cryo-EM maps for CDRH3 and select residues of NorA are superimposed on the structural views (grey mesh). c. Left: NorA (grey) interacting with CDRH3 from Fab25 (top; magenta) and Fab36 (bottom; blue). NorA residues within 4 Å of CDRH3 residues are labeled and displayed in a stick representation (orange). TM2 and TM11 within NorA are hidden for clarity. Right: View from the outward-facing direction and orthogonal to the membrane surface. NorA (grey) is shown in a surface representation with the magenta and blue colors indicating NorA residues within 4 Å of Fab25 CDRH3 or Fab36 CDRH3, respectively. Fab25 and Fab36 are not shown for clarity. d. Left: NorA (grey) interacting with an elongated loop of Fab36 with select residues displayed in stick representation (green). This loop is formed by scaffold residues Leu71 to Arg85 of the Fab light chain variable domain. The CDRH3 loop is shown in ribbon (light blue). TM2 and TM11 within NorA are hidden for clarity. Right: View from the outward-facing direction and orthogonal to the membrane surface. NorA (grey) is shown in a surface representation with the green color indicating NorA residues within 4 Å of the Fab36 light chain scaffold loop. Fab36 is not shown for clarity.
