Extended Data Fig. 2: Validation of single-agent response and protein levels.
From: Systematic identification of biomarker-driven drug combinations to overcome resistance
Sensitivity after 72 hours, as measured by CellTiter-Glo, of a panel of cell lines to each nominated compound, as well as Western blots to assess protein levels. Cell viability values (GR) are normalized to DMSO treatment and to initial (D0) cell number. mRNA expression of each nominated co-target is shown by color (red to blue), along with Pearson correlation coefficients (and associated two-sided P-values) between viability and expression. For Western blots, beta actin (ACTB) or vinculin (VCL) was used as a loading control as indicated to calculate relative protein levels and samples were ordered by their relative compound sensitivity. For (a-e), mRNA expression is also shown below the blots as bar graphs aligned to protein samples and as x-y scatterplots for direct comparison with calculated protein levels.
