Extended Data Fig. 2: Identification of ORF4Tc from Thermobacillus composti and biochemical analysis of its PRP activity.
From: The biosynthetic origin of ribofuranose in bacterial polysaccharides
a, Organization of the glycan biosynthesis gene cluster. The pathway defining wzm/wzt genes (orange) encode the subunits of the ABC transporter. orf4Tc (purple) encodes the dual-domain PRP-gPRT protein. The general functions encoded by the remaining orfs (gray) are predicted by BLAST analyses. orf3 encodes a putative methyltransferase, while orf5 and orf6 appear to encode glycosyltransferases belonging to the GT4 and GT2 families, respectively. b, In vitro reactions containing ORF5KpO4 D10A and ORF4Tc with 2 demonstrate the ability of ORF4Tc to dephosphorylate the ORF5KpO4 D10A product. Products were separated by HPLC, and the product structures are shown on chromatograms with a black square representing the methoxybenzamide tag in 2. The product identities were established by comparison of HPLC retention times to known compounds, authenticated by MS. The analysis was performed in triplicate with similar results.
