Extended Data Fig. 6: Quantification of flavin derived cofactors in CaA producing strains.
From: Engineering cofactor supply and recycling to drive phenolic acid biosynthesis in yeast
a. Representative chromatographs of standards and samples. Total concentration of intracellular FMN(H2) (b), intracellular riboflavin (c) and extracellular riboflavin (d) was detected at different time points. μmol gDCW-1 refers to the number of moles of cofactor per gram of dry cell weight (DCW). All strains were grown at 30 °C in 20 mL of Delft-D medium containing 20 g/L glucose with an initial OD600 of 0.1. All data represent the mean of n = 3 independent biological samples and error bars show standard deviation. Statistical analysis was performed by using Student’s t test (one-tailed; two-sample unequal variance; *p < 0.05, **p < 0.01, ***p < 0.001).
