Extended Data Fig. 9: Quantification of cellular SAM and SAH in FA producing strains. | Nature Chemical Biology

Extended Data Fig. 9: Quantification of cellular SAM and SAH in FA producing strains.

From: Engineering cofactor supply and recycling to drive phenolic acid biosynthesis in yeast

Extended Data Fig. 9: Quantification of cellular SAM and SAH in FA producing strains.

a. Representative chromatographs of standards and samples. Corresponding mass spectra of SAM (b) and SAH (c). d. Cellular SAM/SAH ratios were detected at different time points. All strains were grown at 30 °C in 20 mL of Delft-D medium containing 20 g/L glucose. All data represent the mean of n = 3 independent biological samples and error bars show standard deviation. Statistical analysis was performed by using Student’s t test (one-tailed; two-sample unequal variance; *p < 0.05, **p < 0.01, ***p < 0.001).

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