Extended Data Fig. 4: Optimization of HPAB/C and the shikimic acid pathway for CaA production.
From: Engineering cofactor supply and recycling to drive phenolic acid biosynthesis in yeast
a. Overview of the yeast metabolic pathway for CaA biosynthesis. ARO1 encoding shikimate dehydrogenase, ARO2 encoding chorismate synthase, ARO3 encoding DAHP synthase, MtPDH1 encoding Medicago truncatula prephenate dehydrogenase, PHA2 encoding S. cerevisiae prephenate dehydratase 2. See Supplementary Fig. 5 legend regarding other abbreviations. The fusion EcHPAB-EcHPAC (a) and PaHPAB-SeHPAC (b) result in a negative effect on CaA production. PaHPAB, encoding Pseudomonas aeruginosa HpaB; SeHPAC, encoding Salmonella enterica HpaC; EcHPAB: encoding E. coli HpaB; EcHPAC: encoding E. coli HpaC. d. Further optimization of the shikimic acid pathway increased CaA titers with the CYP system. Cells were grown in defined minimal medium containing 20 g/L glucose and cultures were extracted after 96 h of growth for phenolic acid detection. All data represent the mean of n = 3 independent biological samples and error bars show standard deviation. All data represent the mean of n = 3 independent biological samples and error bars show standard deviation. Statistical analysis was performed by using Student’s t test (one-tailed; two-sample unequal variance; *p < 0.05, **p < 0.01, ***p < 0.001).
