Extended Data Fig. 4: Dissociation of probe 3 from recombinant AURKA and Src.
From: Reversible lysine-targeted probes reveal residence time-based kinase selectivity
AURKA or Src (5 μM) was treated with probe 3 (5.1 μM) in 50 mM HEPES, pH 8.0 at RT for 5 min, followed by 20-fold dilution into buffer containing 10 μM XO44. The percentage of XO44-modified and unmodified kinase was quantified by LC-MS at the indicated time points, and % unmodified kinase (corresponding to probe 3-bound kinase) was plotted vs. time (n = 2, mean values from two independently performed experiments were plotted).
