Extended Data Fig. 4: Type I PRMT inhibition stimulates interferon responses in a subset of TNBC cell lines.
From: PRMT inhibition induces a viral mimicry response in triple-negative breast cancer
a. Flow cytometry cell cycle analysis for MDA-MB-468 cells cultured with or without MS023 for 5 days (means±s.d., n = 3, two-way ANOVA with Dunnett’s test for multiple comparisons, ns: not significant). b. GSEA of the apoptosis pathway in MDA-MB-468 cells after MS023 treatment for 5 days. (one-tailed Fisher’s exact test for multiple comparisons). c. Apoptotic cell counts of MS023 treated for 5 days by caspase 3/7 staining (means±s.d., n = 3, two-sided Student’s t test). d. Volcano plot of log2 fold change for genes significantly upregulated (red in the right panel) or downregulated (blue in left panel) upon MS023 treatment (n = 3) in Hs578-T cell line (unpaired two-tailed Student’s t-test). e. Gene set enrichment analysis (GSEA) of all ranked differential expressed genes in Hs578-T after 5 days MS023 treatment (one-tailed Fisher’s exact test for multiple comparisons). f. Growth curves of MDA-MB-468 cell with indicated concentration of MS023 for 2 days culture (means±s.d., n = 4). g. Top 10 gene set enrichment analysis (GSEA) of all ranked differential expressed genes in MDA-MB-468 after 2 days MS023 treatment. h. Representative immunoblots showing DNA damage after MS023 treatment for 2 days (one of three independent experiments are shown). i. Quantification of cytoplasmic dsRNA signal intensity in MDA-MB-468 cells with indicated compound treatment for 2 days (means±s.d., n = 3 independent biologically experiments of at least 86 cells per group, one-way ANOVA with Dunnett’s test for multiple comparisons).
