Fig. 6: MS023 activates dsRNA sensors to induce IFN signaling.
From: PRMT inhibition induces a viral mimicry response in triple-negative breast cancer
a, The expression of IFIH1 and indicated IFN-responsive genes in scramble-treated (shCTL), MS023-treated and IFIH1-knockdown/MS023-treated MDA-MB-468 cells analyzed by qRT–PCR. b, The expression of DDX58 and indicated IFN-responsive genes in scramble-treated, MS023-treated and DDX58-knockdown/MS023-treated MDA-MB-468 cells analyzed by qRT–PCR. c, The expression of TLR3 and indicated IFN-responsive genes in scramble-treated, MS023-treated and TLR3-knockdown/MS023-treated MDA-MB-468 cells analyzed by qRT–PCR. Data in a–c are shown as mean ± s.d. (n = 3) and were analyzed by one-way ANOVA with Dunnett’s test for multiple comparisons. d, Cell confluence of scramble-treated, IFIH1-knockdown, DDX58-knockdown and TLR3-knockdown MDA-MB-468 cells. Data are shown as mean ± s.d. (n = 4) and were analyzed by one-way ANOVA with Dunnett’s test for multiple comparisons. e, Cell confluence of scramble-treated, MS023-treated and IFIH1-knockdown/MS023-treated MDA-MB-468 cells. f, Cell confluence of scramble-treated, MS023-treated and DDX58-knockdown/MS023-treated MDA-MB-468 cells. g, Cell confluence of scramble-treated, MS023-treated and TLR3-knockdown/MS023-treated MDA-MB-468 cells. Data in e–g are shown as mean ± s.d. (n = 4) and were analyzed by two-way ANOVA with Dunnett’s test for multiple comparisons. h, Bar plot showing the number of ASEs belonging to each of the main alternative splicing categories. i, Bar plot showing the count of IR pairs in which the intron intersects with only the first Alu in the pair (1; white), with only the second Alu in the pair (2; gray) or with the two Alus in the pair (1/2; red). j, qRT–PCR analysis of the indicated genes after J2 immunoprecipitation. Data are shown as mean ± s.d. (n = 4) and were analyzed by one-way ANOVA with Dunnett’s test for multiple comparisons; ssRNA, single-stranded RNA.
