Extended Data Fig. 2: Mucin glycan-mediated suppression of virulence pathways is independent of specific experimental conditions, including medium and time point.
From: Mucin O-glycans are natural inhibitors of Candida albicans pathogenicity
a) Quantification of C. albicans morphology in the presence of mucins at 8 h using phase-contrast images from n = 3 (MUC5AC) or n = 4 (Media, MUC5B, MUC2) biologically independent replicates. b) C. albicans SC5314 cells were diluted into the specified medium with or without mucin glycans and cultured at 37 °C. Phase-contrast images of C. albicans were acquired after 8 h in the presence or absence of mucin glycan libraries purified from MUC5AC. Scale bar, 20 μm. c) C. albicans SC5314 cells were diluted into the specified medium with or without mucin glycans and cultured at 37 °C. Phase-contrast images of C. albicans were acquired after 4 h in the presence or absence of mucin glycan libraries purified from MUC5AC. Scale bar, 20 μm. d) Quantification of C. albicans morphology in the presence of mucin glycans in RPMI medium at 8 h using phase-contrast images from n = 6 (CPH1), n = 4 (WT), n = 3 (RAS1) biologically independent replicates. prADH1-CPH1 strain abbreviated as CPH1; RAS1G13V strain abbreviated as RAS1. e) Quantification of C. albicans morphology in the presence of mucin glycans in Spider medium at 8 h using phase-contrast images from n = 3 (WT), n = 4 (EFG1-) or n = 5 (EFG1+) independent biological replicates. PCKpr-efg1-T206E strain abbreviated as EFG1. f) Quantification of C. albicans morphology in the presence of synthesized glycans in RPMI medium at 8 h using phase-contrast images from n = 6 (MG), n = 3 (1, 3, 4, 6) biologically independent replicates. 1, Core 1; 3, Core 1+fucose; 6, Core 2+galactose; 4, Core 1+sialic acid. For b,c, similar results were observed in different fields of view across three biologically independent replicates. For a,d,e,f, the percentage of hyphae was obtained by dividing the number of hyphae by the total number of cells from n > 100 cells. Bars indicate mean ± s.e.m. MG, mucin glycans.
