Extended Data Fig. 5: PITPα/β knockout induces YAP phosphorylation independent of cell types.
From: Hippo pathway regulation by phosphatidylinositol transfer protein and phosphoinositides
a, Genomic characterization of the PITPβ knockout cell clone #1.12. The HEK293A cell line has three alleles of PITPβ. DNA sequence confirms mutations of the three alleles. b, The PITPα siRNA knockdown efficiency was measured by Quantitative Real-time PCR. Results are mean ± SD of three technically replicates. c, PITPα/β knockout induces YAP phosphorylation independent of cell types. These knockout cell pools were generated by co-infecting lentiviruses stably expressing Cas9 and guide RNA targeting PITPNA and PITPNB. Cells were lysed and subjected to Western Blot with indicated antibodies. d, PITPα/β knockout blocks LPA-induced YAP de-phosphorylation. HEK293A wild-type and PITPα/β dKO cell pool were treated with Latrunculin B or LPA and then analyzed by Western blot with indicated antibodies.
