Extended Data Fig. 6: VT01454 preferentially depletes plasma membrane PI4P.
From: Hippo pathway regulation by phosphatidylinositol transfer protein and phosphoinositides
a, Volume (combined) view of the Z-section confocal images in Fig. 5a. Scale bar = 10 μm. b, Zoomed out view of area with multiple cells to indicate that the single cell images in Fig. 5a are representative. Scale bar = 10μm. c, VT01454 treatment preferentially depletes plasma membrane PI4P. Scale bar = 10 μm. d, VT01454 has little effect on PI3P. HEK293A cells were transfected with RFP-p40px, which is a reporter for PI3P, then treated with VT01454. Scale bar = 10 μm. e, Expression of Sac1-GRIP does not induce YAP cytoplasmic translocation. Scale bar = 20 μm.
