Extended Data Fig. 7: PI4KA overexpression dampens the effect of VT01454.
From: Hippo pathway regulation by phosphatidylinositol transfer protein and phosphoinositides
a, Inhibition of PI4KA increases YAP cytoplasmic localization. HEK293A cells were treated with PI4KA inhibitor GSK-A1 or PI4KB inhibitor PI4KIIIb-IN-10 prior to YAP staining. Scale bar = 20 μm. Quantification is shown on the right. b, GSK-A1 stimulates phosphorylation of LATS and YAP. c, PI4KA overexpression dampens the effect of VT01454 on YAP phosphorylation. Flag-YAP was co-expressed with HA-PI4KA or vector in HEK293A cells. After 24 hours, these cells were treated with DMSO or VT01454 for 1 hour, then Western blotted with the indicated antibodies.
