Extended Data Fig. 8: NF2 is required for the phosphorylation of LATS1 and YAP induced by VT01454.
From: Hippo pathway regulation by phosphatidylinositol transfer protein and phosphoinositides
a, Characterization of FERM protein (Protein 4.1/ Ezrin/ Radixin/ Moesin). knockout efficiency in HEK293A. The knockout cell pool was generated with individual or combinatory lentivirus stably expressing Cas9 and specific guide RNAs. These cells were selected with puromycin for 48 hours before subjecting to western blot with indicated antibodies. b, Knockout of Protein 4.1, Ezrin, Radixin, and Moesin did not affect YAP phosphorylation. The cells as described in panel a were treated with DMSO or 20 nM VT01454 for 1 hour before western blot with indicated antibodies. c, NF2 is required for the phosphorylation of LATS1 and YAP induced by VT01454. The wild-type or NF2 knockout cells were treated with 20 nM VT01454 for indicated times before subjecting to western blot with indicated antibodies.
