Extended Data Fig. 1: MCB induce YAP inactivation, phosphorylation and cytoplasmic localization.
From: Hippo pathway regulation by phosphatidylinositol transfer protein and phosphoinositides
a, MCB inhibits the YAP transcription reporter. HEK293A clone, which has the 8X TBD-luciferase reporter stably integrated in the genome, was treated with increasing concentration of MCB. Luciferase activity was determined to measure YAP transcriptional activity. n = 4 biologically independent samples. b, MCB induces YAP phosphorylation in a dose-dependent manner. HEK293A cells were treated with indicated concentration of MCB for 2 hours. Western blot was performed with indicated antibodies. c, MCB induce YAP phosphorylation in a time-dependent manner. HEK293A cells were treated with MCB (270 nM) for the indicated times. Cell lysates were analyzed by Western blot with indicated antibodies. d, MCB induces YAP phosphorylation in 92.1 cells. Cells were treated with 0.7 μM MCB for the indicated times. e, MCB induces YAP cytoplasmic translocation. HEK293A cells were treated with 270 nM MCB for 2 hours and subjected to immunofluorescence for YAP localization. Scale bar = 10 μm. The right panel shows quantification of a representative experiment.
