Extended Data Fig. 8: MDH1 glycosylation promotes PDAC cell proliferation in part through redox regulation.
From: O-GlcNAcylation promotes pancreatic tumor growth by regulating malate dehydrogenase 1
a, Relative cell proliferation of SW1990 cells reconstituted with WT or S189A MDH1, in the presence or absence of ME1 depletion and malate (n = 5 independent assays). b, Immunoblotting of SW1990 cells reconstituted with WT or S189A MDH1, in the presence or absence of ME1 depletion and malate. c, Relative cell number of SW1990 cells reconstituted with WT or S189A MDH1 after treatment with 5 mM NAC, 5 mM GSH or 4 mM cell-permeable malate for 24 h (n = 5 independent assays). d, Cell survival rate of SW1990 cells reconstituted with WT or S189A MDH1 after treatment with indicated concentrations of H2O2 for 1 h (n = 5 independent assays). e, Relative cell number of SW1990 cells reconstituted with WT or S189A MDH1 after incubation with 4 mM cell-permeable malate for 12 h, followed by treatment with 0.3 mM H2O2 for 16 h (n = 5 independent assays). Error bars of data in a, c, d and e denote the means ± SD. Statistical analyses were performed by two-tailed Student’s t-test.
